The dorsal skinfold chamber: studying angiogenesis by intravital microscopy

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Publication Details

Author list: Sckell A
Publication year: 2009
Volume number: 467
Start page: 305
End page: 317
Number of pages: 13
Languages: English-Great Britain (EN-GB)


Abstract

Intravital microscopy represents an internationally accepted and
sophisticated experimental method to study angiogenesis,
microcirculation, and many other parameters in a wide variety of
neoplastic and nonneoplastic tissues. Since 1924, when the first
transparent chamber model in animals was introduced, many other chamber
models have been described in the literature for studying angiogenesis
and microcirculation. Because angiogenesis is an active and dynamic
process, one of the major strengths of chamber models is the possibility
of monitoring angiogenesis in vivo continuously for up to several weeks
with high spatial and temporal resolution. In addition, after the
termination of experiments, tissue samples can be excised easily and
further examined by various in vitro methods, such as histology,
immunohistochemistry, and molecular biology. This chapter describes the
protocol for the surgical preparation of a dorsal skinfold chamber in
mice as well as the method to implant tumors in this chamber for further
investigations of angiogenesis and other microcirculatory parameters.
However, the application of the dorsal skinfold chamber model is not
limited to the investigation of neoplastic tissues. To this end, the
investigation of angiogenesis and other microcirculatory parameters of
nonneoplastic tissues such as tendons, osteochondral grafts, or
pancreatic islets have been objects of interest.


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Last updated on 2019-13-08 at 00:15